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English words and Examples of Usage
use "cycled" in a sentence
It was below zero this morning, but I cycled to school.
Richard and Jennifer cycled across France for their holidays last summer.
The grade of the hill we cycled up was 14%.
It was a total killer.
I also recall with affection the late John Leaver, who cycled around the English countryside as a boy collecting pub-names.
The supernatant fractions were pooled, passed through a pre-cycled CM-52 column and eluted with a gradient of 0.
All plasmid inserts were amplified in 100 µl reactions using the following primer sequences: M13 Forward 5'-GTTTTCCCAGTCACGACGTTG-3' M13 Reverse 5'-TGAGCGGATAACAATTTCACACAG PCR reactions were cycled 35 times under the following conditions: 94 degrees for 30 seconds, 55 degrees for 1 minute and 72 degrees for 2 minutes.
The reaction was cycled 44 times at 94°C for 1 min, 35°C for 1.
In vitrotranscription The introns were PCR-amplified with KM37 (GAT AAT ACG ACT CAC TAT AAT GGC ATT ACC GCC TTG T) and GM24 (GCT CTA GAC TTA GCT ACA ATA TGA AC) in 25 µl reactions under the conditions stated above and cycled 20 times.
The agarose plug was added to a fresh PCR reaction (100 µl) and cycled 15 times, DNA was purified using a QIAquick kit and quantitated.
15 pmol of nt 1932 to nt 1959 primer and 15 pmol of nt 3100 to 3081 primer were added and further cycled for 4 minutes at 95°C, then 30X of 2 minutes at 95°C, 1 minute at 60°C, 1 minute at 72°C and finally 10 minutes at 72°C.
These reactions were carried out in 50 µ l using 5 pmol of each primer, and were cycled with a 9600 GeneAmp System (Perkin Elmer, Emeryville, CA, USA) as follows: denaturation at 94°C for 40 s, annealing at 65°C for 45 s, and extension at 72°C for 40 s.
The reactions were carried out in 50 µl using 5 pmol of each primer and cycled as follows: denaturation at 94°C for 45 s, annealing at 65°C for 45 s, and extension at 72°C for 45 s.
The reactions were carried out in 25 µ l and cycled as follows: denaturation at 94°C for 30 s, annealing at 52°C for 45 s, and extension at 72°C for 30 s.
Downstream processing currently carries out this validation of the annotations in bulk, but this delays feedback and problems with the peptides are only cycled back to the curators for correction after some time has passed.
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