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Example Sentences for "antisera"

Co-immunoprecipitation experiments using affinity purified rabbit antisera confirmed these interactions at the level of the endogenous proteins.

While each of the five antisera co-precipitated at least one of the other subunits, Pip1p, Pop1p, and Pop2p antisera co-precipitated all five proteins from wild-type cell lysate (Fig.

Antibodies Rabbit antisera were raised at Josman LCC (Napa, CA) against bacterially expressed MBP-Psh1p, GST-Pcu1p, GST-Pip1p, and GST-Pop1p.

Rabbit antisera against Pop2p and Rum1p were described before [ 20 ] .

This species reacted with both anti-MHCK-C antisera and with anti-FLAG epitope antibodies (not shown), indicating this fragment to be an amino-terminal domain of MHCK-C.

Louis), and the secondary antisera was horseradish peroxidase-conjugated sheep anti-mouse IgG (dilution 1:1000 in PBS-T, Amersham, Arlington, IL).

The antisera against Tbp1 and Tbp2 killed the homologous as well as majority of heterologous strains examined, with varying efficiency [ 30 ] .

In the Drosophila nervous system mab 3D2 produces exactly the same staining pattern as that observed with polyclonal antisera against the ARD nAChR subunit [ 25].

The cross-reactivities of the genistein and daidzein antisera with other isoflavone metabolites have been described previously [ 60 ] .

Blocking of neurotoxicity of supernatants from HIV infected monocytes with antisera to gp120 and Tat Cell culture supernatants from HIV infected monocytic cells were collected periodically and analyzed for their neurotoxic potential.

Complete obliteration of neurotoxicity was noted when the supernatants were immunoabsorbed against antisera to gp120 and Tat (Figure 4B).

However, when immunoabsorbed against each of the antisera alone, the effect on neurotoxicity of the culture supernatants was not significant (not shown).

Receptor immunoreactivity was determined using monoclonal or polyclonal antisera as listed in Table 1.

Following three washes in PBS the cells were incubated with fluorochrome conjugated secondary antisera diluted in blocking buffer (Table 1) for one hour at room temperature.



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