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Sentences for "aortas"
Hearts and abdominal aortas were collected and histopathological and immunohistochemical analyses were performed.
Histology H-E, Movat trichrome, and Verhoeff van Gieson, staining was performed on 4 µm sections of aortas fixed for 16 hours in neutral buffered formalin (10%), and embedded in paraffin.
Vascular disease in the IFN-?
R-/- group of mice began with development of an inflammatory infiltrate in the adventitia of aortas 3-4 weeks after infection in 30% of animals (Table 1) (Fig.
On the contrary, in the IFN-?
R-/- mice infected with MCMV, vascular disease progressed to the formation of neointima in aortas by 4 months of age, (Fig.
Histopathological analysis of abdominal aortas collected from transgenic, infected and irradiated animals revealed severe vasculitis characterized by significant inflammatory infiltrate in the adventitia and media (Table 2), (Fig.
The most striking feature of the MCMV induced vascular disease was the formation of neointima in aortas of MCMV infected immunodeficient mice after irradiation (Table 2), (Fig 2).
Verhoeff-van Gieson staining of aortas collected from IFN-?
R-/- mice 30 days after irradiation (not shown) and IFN-?
R-/- mice 60 days after irradiation (Fig.
We utilized monoclonal antibody specific for ?
-SMA, a marker for myofibroblast cells, and found that neointima seen in the aortas of MCMV infected experimental animals contained myofibroblasts.
Immunohistochemical characterization of immune response to MCMV infection Immunohistochemical characterization of the inflammatory infiltrate seen in the adventitia and media of the affected aortas revealed both helper T-lymphocytes (CD4 +) (Fig.
Second, abdominal aortas from iron deficient rats display not only increased diameters, but also significant increases in distensibility.
Experiment 2 Representative aortas from control and iron deficient rats after one month on the respective diets are shown in figure 5, and mean aorta external diameter at 100 mmHg pressure is illustrated in figure 6.
Overall, the diameter of aortas from iron deficient rats was greater than from control rats (*, p = 0.
0101) with the iron deficient aortas more compliant than the controls overall.
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