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Sentences for "apheresis"
As a supportive care, leukodepleted (containing <10 5leukocytes per one transfusion unit) irradiated (50Gy) blood products (packed red blood cells, platelets obtained by apheresis from one donor) were given only.
Contrary to the in vivo observation, however, in vitro stimulation with IL-2 (6,000 IU/ml) of PBMC obtained from apheresis of melanoma patients did not alter mononuclear cell counts, suggesting that cell death is not a direct effect of IL-2 (Figure 1b).
A substantial difference between gradient-separated PBMC and circulating cells in vivo is the lack of plasma, platelets and polymorphonuclear cells as a result of the apheresis process.
(PBMC from apheresis used as reference for in vivo studies and as source of cells for in vitro studies are highly enriched in monocytes and lymphocytes, as seen in Figure 1b.
PBMC were obtained from each patient by apheresis before IL-2 infusion and at 3 h after the fourth dose of IL-2 (patients H.
) by apheresis before administration of IL-2 were seeded at 6 × 10 6per well of a 24-well plate in OPTI-MEM medium without serum supplementation and were incubated at 37°C in the presence or absence of 6,000 IU/ml IL-2 for 0 or 5 min, or 1, 2, 3, 4, 6 and 8 h.
Each probe/test sample (antisense RNA (aRNA) obtained at each time point) was labeled with Cy5 and mixed with Cy3-labeled reference sample (non-stimulated PBMC obtained from apheresis before IL-2 administration).
Each probe/test PBMC sample was labeled with Cy5 and mixed with Cy3-labeled reference sample obtained from apheresis before IL-2 administration.
Differential stimulation of PBMC with IL-2 for supernatant collection PBMC collected from apheresis before IL-2 treatment were obtained from one patient (L.
Methods Differentiation of Primary Human Macrophages Peripheral blood mononuclear cells were isolated from whole blood of healthy donors following apheresis and elutriation.
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