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And, most importantly, he recommends buying and selling complex arrays of options, often at the same time.
Many enzymes form ordered arrays of multimolecular complexes in which a substrate is progressively passed from one to another active site along an analogy to an assembly line.
Expression arrays that pave the entire target genome with probes can detect novel transcripts [ 41 ] , and cDNA libraries that enrich for small, nonpolyadenylated RNAs can be constructed and EST sequenced [ 42 ] .
Presently, two technologies dominate the field of high-density microarrays: cDNA arrays and oligonucleotide arrays.
The probe arrays were scanned at 560 nm using a confocal laser-scanning microscope (made for Affymetrix by Hewlett-Packard).
Oligonucleotide arrays are either spotted or constructed by chemically synthesizing approximately 25-mer oligonucleotide probes directly onto a glass or silicon surface using photolithographic technology [ 3 ] .
There are multiple probe pairs for each mRNA (8-20 for the arrays used in the present study).
Microarray Suite also has a procedure ("comparative analysis" algorithm) for comparing two arrays at the level of individual probe pairs.
, 9 comparisons for 3 arrays per group) and report the average of the ratios as the change in gene expression [ 1 2 3 4 5 9 ] .
However, with 6 arrays per group, even a single outlier can markedly reduce the value of t even when there is no overlap between groups.
Results Normalization method Comparisons among arrays are meaningful only after accounting for variability in overall target concentration ("target" is the Affymetrix nomenclature for a labeled cRNA that hybridizes with a probe), hybridization efficiency, staining, etc.
The trimmed mean was 649 ± 14 (standard deviation) arbitrary units for the 6 arrays probing RNA from young muscle, and 643 ± 18 for the 6 arrays probing RNA from older muscle.
408 more results not shown.
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