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Sentences for "assaying "
Progress in research on silkworm receptors for Bt toxins has provided a means for assaying mutant toxins with potentially altered binding and activity.
PP5 activity was followed during the purification by assaying for trypsin-stimulated phosphohydrolase activity at 37°C vs.
As described under Materials and Methods, the purification was in fact monitored by assaying for a trypsin-activated phosphatase with the assumption that PfPP5 may behave like mammalian PP5 in this regard [ 18 20 21 ] .
PP5 activity was followed during the purification by assaying trypsin-stimulated pNPPase activity.
Plasmids A reporter construct containing the basal promoter of the rat RIIß 5'-flanking region (-394 to -123) upstream of a CAT-reporter gene (pCATbasic, Promega, Madison, WI) was used for assaying RIIß promoter activity [ 18 ] .
The fusion proteins were tested for function by assaying their abilities to stimulate adenylyl cyclase in response to receptor stimulation.
While the anti-tumor effect of the combinatorial treatment does not completely resolve tumor burden and the mice eventually succumb, it is important to recognize that the Rip1-Tag2 mice are a very stringent system for assaying immunotherapies because of the multifocal nature of tumor progression in these mice.
Although these numbers are higher than the 1 in 15,000 figure reported for SuperScriptII (Taurai Nenguke, personal communication), the in vitro assay used to obtain this error rate is based on assaying a single site for mutations that revert an amber codon.
Testing specific genes by overexpression in transgenic flies is a candidate gene approach, meaning that the investigator must make an educated guess as to which genes are worth assaying by this relatively labor-intensive method.
Progress has been made recently in assaying RNAi effects in both plant [ 66 ] and animal [ 67 ] parasitic nematodes.
LPS preparations were diluted using the PG-1 vehicle prior to assaying with PG-1.
By this process the metal was divided into convenient portions, of any degree of smallness, and bearing a recognized proportion to one another; and the trouble was saved of weighing and assaying at every change of possessors-an inconvenience which, on the occasion of small purchases, would soon have become insupportable.
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