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Example Sentences for "assays "

Caspase activity assays Cells (10 6per 60 mm dish) were grown for 16 h.

Similar antibody assays were not performed to substantiate the identity of the purified APN because it clearly displayed strong, characteristic APN enzyme activity.

mori that used BBMV assays or used purified receptors in ELISA assays or blots, however, no Cry toxin studies concerning B.

mori and BBMV binding assays were conducted as described [ 4 ] .

mori BBMVs and 40 µl of candidate receptor fractions were blotted onto PVDF membrane and assays was carried out as reported previously [ 27 ] , except for using 1:1000 anti-Bt-R 1 polyclonal antiserum.

Cell adhesion assay Cell adhesion assays were performed essentially as described [ 36].

Binding assays showed that overexpressed HA-tagged Pip1p specifically associated with co-overexpressed 6xHis-Myc-tagged Ubc1p, Ubc7p, Ubc11p, Ubc12p, Ubc13p, Ubc14p, and Mms2p.

Several different 5'-end labeled substrates were prepared and used in cleavage assays to compare the cleavage sites selected by Top67 versus topoisomerase I.

DNA cleavage assay The cleavage assays were carried out with 1 pmole of 5' 32P-end labeled DNA substrate and 5-10 pmoles of topoisomerase I or Top67 in 10 µl of the buffer used for the gel mobility shift assay.

Inhibition binding assays were also conducted.

Binding assays Transfected cells were scraped from the dishes, washed twice with Dulbecco's PBS (New Life Technologies, NY), then resuspended in 1.

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