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Example Sentences for "astrocyte "

The advantage to using astrocyte lines is that many of these lines can maintain a monolayer in culture for long periods of time [ 6 ] .

Results Characterization of tkT lines We have previously characterized clonal mouse astrocyte cell lines, immortalized by transduction of SV40 large T antigen expressed from the MoMuLV LTR [ 6 13 ] .

The astrocyte lineage of the LTR lines has been published [ 6 13 ] .

The astrocyte lineage of the tkT lines was confirmed by the presence of the astrocyte-specific intermediate filament protein, GFAP [ 14 ] (Figure 1).

All of the lines contained GFAP, whereas NIH 3T3 cells treated with dibutyryl cAMP at the same level as the astrocyte lines did not.

To test this idea, we measured Tag expression by immunofluorescence flow cytometry (see Figure 4) in 6 Tag-immortalized mouse astrocyte lines plated at a range of cell densities and cultured for 2-3 days as previously described [ 15 ] .

However, when a large number of htk-Tag astrocyte clones were checked 100 days after creation, the expression levels displayed a very large range, and thus we expect that this weak promoter is subject to positional effects on transcription or mutational events that affect gene expression (unpublished data).

Materials and Methods Generation of astrocyte cell lines Cortical astrocytes were harvested from neonatal C57BL/6 mice and infected with a replication-defective retrovirus encoding Tag and neomycin resistance as previously described [ 13 ] .

To test for astrocyte lineage, early passage cells were grown in dibutyryl cAMP (Sigma) to enhance GFAP expression.

The present study shows that GC's can play a critical role in hindering astrocyte development in the absence of LIFR as seen through the partial abrogation of this defect following exposure to RU486 in Lifr -/- mice.



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