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Sentences for "att "
The Duke, who was then Viceroy of Ireland, replied, 'You will let me know whether ye application is to be made to Lord Orford, who is ye Ranger of ye Park, or to ye King himself: in ye latter case I would write to Lord Sydney att ye same time; if it be to the King a greater object might be easier accomplished than this trifle, as I know he is very particular about his Parks; at least he is so about St.
For NRG1, the oligos used were HZ034, 5' TCG ACC AGC ATA TTA CTA CCC TTC GCA AAC TTT CAG GCA CTG TGC GGT ATT TCA CAC CG 3', and HZ035, 5' GTA GTA CTG CTA ATG AGA AAA ACA CGG GTA TAC CGT CAA AGA TTG TAC TGA GAG TGC AC 3'.
For MIG2, the oligos were HZ045, 5' TGA CCT CGA GAA CAA ACA AAA TAA AAA TAA AAA AAG AGA CTG TGC GGT ATT TCA CAC CG 3', and HZ046, 5' TTA GAG GAA AAA TGG TGA GAT AAA AAG GGG CCG TAA AGG AGA TTG TAC TGA GAG TGC AC 3'.
Primer sequences were: IL-7 [ 65 ] : IL-7 (5') 5'ACT ACA CCC ACC TCC CGC A3', IL-7 (3') 5'TCT CAG TAG TCT CTT TAG G3', SCF [ 65 ] : SCF (5') 5'TCT TCA ACT GCT CCT ATT T3', SCF (3') 5'ACT GCT ACT GCT GTC ATT C3', TGFß1 [ 66 ] : TGFß1(5') 5'GCG GAC TAC TAT GCT AAA GAG G3', TGFß1(3') 5'GTT GTG TTG GTT GTA GAG GGC A3', ß-actin [ 67 ] : ß-actin (5') 5'GGG TCA GAA GGA CTC CTA TG3', ß-actin (3') 5'GTA ACA ATG CCA TGT TCA AT3'.
2: CTA G AG GGG AAT TGT TAT CCG CTC ACA ATT CCC CTA TAG TGA GTG GTA TTA ATT TCG CGG GAT CGA (XbaI site in bold) Similarly, oligonucleotides gcP1.
66: CTA G AG GGG AAT TGT TAT CCG CTC ACA ATT CCC CTA TAG TGN NNN GTA TTA ATT TCG CGG GAT CGA (XbaI site in bold, N indicates a random sequence region) In the selection for T3-like promoter specificity, T7 RNA polymerase was randomized at amino acid positions 746-748.
129 (TAA TCT TAC CCC GGA ATT ATA TCC AGC TGC ATG TCA CCA TGC AGA GCA GAC TAT ATC TCC AAC TTG TTA AAG CAA GTT GTC TAT CGT TTC GAG TCA CTT GAC CCT ACT CCC CAA AGG GAT AGT CGT TAG).
24 (CCC GGA ATT CTA TCC AGC TGC ATG, 0.
C TTG GCA GTA AAT GCC TAA CGA CTA TCC CTT, Th5P6 GCC TGA GTA TAA GGT GAC TTA TAC TTG TAA TCT ATC TAA ACG GGG AAC CTC TCT AGT AGA CAA TCC CGT ATA CCA GCA TCG TCT TGA TGC CCT TGG CAG CTA ACG ACT ATC CCT T, Th6P6 GCC TGA GTA TAA GGT GAC TTA TAC TTG TAA TCT ATC TAA ACG GGG AAC CTC TCT AGT AGA CAA TCC CGT GAT ACC AGC ATC GTC TTG ATG CCC TTG GCA GCC TAA CGA CTA TCC CTT, Th1P5 TGA GTA TAA GGT GAC TTA TAC TAG TAA TCT ATC TAA ACG GGG AAC CTC TAT ACC AGC ATC GTC TTG ATG CCC TTG GCA GAG ACA ATC CCG TGC TAA ATT GTA GGA CTG CCC GGG TTC TAC ATA AAT GCC TAA CGA CTA TCC CTT, Th2P5 TGA GTA TAA GGT GAC TTA TAC TAG TAA TCT ATC TAA ACG GGG AAC CTA TAC CAG CAT CGT CTT GAT GCC CTT GGC AGA CAA TCC CGT GCT AAA TTG TAG GAC TGC CCG GGT TCT ACA TAA ATG CCT AAC GAC TAT CCC TT, 3Mex2P6 GTA ATC TAT CTA AAC GGG GAA CCT CTC TAG TAG ACA ATC CCG TGC TAA ATT GAT ACC AGC ATC GTC TTG ATG CCA TTG GCA GCA TAA ATG CCT AAC GAC TAT CCC TT, and Th2P6.
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