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Example Sentences for "autoradiography "

Conversion of p27 into polyubiquitylated high molecular weight species was monitored by autoradiography of 35S-labeled p27 following gel electrophoresis.

Phosphorylated proteins were detected by autoradiography or quantified on a PhosphoImager.

The immunoprecipitates were subjected to SDS-PAGE followed by autoradiography to examine eIF-2 phosphorylation.

Before the availability of antibodies against nicotinic acetylcholine receptors of dipteran flies, cholinergic pathways have been analyzed by more indirect methods, mainly by immunocytochemistry against choline acetyltransferase (ChAT), an enzyme of the acetylcholine metabolism [ 38, 39] or autoradiography of 125I-?

-bungarotoxin binding [ 40, 41].

Following electrophoresis, gels were dried for 2 hours at 80°C and subjected to autoradiography for 24 hours at -70°C.

The phosphorylated histone H1 was resolved by SDS-PAGE and detected by autoradiography using Kodak XAR-5 film.

Fractionation by SDS-polyacrylamide gel electrophoresis and autoradiography of the resulting immunocomplexes from these cells showed crosslinking exclusively to HA-Smo, but with an efficiency of less than 1% (data not shown).

Hybridization was visualized by autoradiography with X-ray film (Kodak) at -80°C.

Cyclin E variants in Cdk2 IP were detected by probing with HE12 primary antibody (PharMingen), followed by goat anti-mouse IgG and IgM (Jackson Immunoresearch) secondary antibody conjugated with horse radish peroxidase, and visualized by a chemiluminescent detection system (ECL, Amersham Pharmacia Biotechnology) and autoradiography as indicated above.

After an autoradiography and counterstaining, the sections were analyzed microscopically.

In situbinding autoradiography The method of Hub and Rot [ 19 ] was followed, with minor modifications (see Supplementary Material).

For direct comparison, each chemokine was added at the same specific radioactivity and concentration, and autoradiography was performed for an identical exposure time.

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