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Disruption of the ifkAgene results in earlier aggregation and formation of larger than normal mounds BS153 cells, possessing a disruption of the ifkA gene, grew slightly slower than the parental Ax4 cells in axenic broth, doubling in 10 hour instead of 8 hours (data not shown).
Methods Cell growth and development The axenic strain, Ax4 [ 44 ] , was used as the parental or "wild type" strain in all experiments.
Distributions of GC content are approximately normal in two of three cases studied, those of axenic P.
This is a considerably greater number than for axenic P.
The axenic and nodulating root libraries had the smallest portion of putative microbial transcripts (< 2%, Figure 4c), with the axenic library closely resembling nodulating root libraries.
The predicted portion of microbial transcripts was greater in the axenic root-hair enriched library than in mixed cultures.
Analysis of another axenic root-hair enriched library, particularly one provided a carbon source to identify potential contaminants, and not treated with an inhibitor of ethylene response, would be an informative test.
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