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(c) Locomotive movement, or true motility, is determined by observing some one particular bacillus changing its position in the field independently of, and in a direction contrary to, other organisms present.
For example, under one set of conditions the examination of a pure cultivation of a bacillus may show a short oval rod as the predominant form, whilst another[Pg 134] culture of the same bacillus, but grown under different conditions, may consist almost entirely of long filaments or threads.
The shape of the parent cell may be unaltered, as in the anthrax bacillus, or altered, as in the tetanus bacillus, and these points serve as the basis for a classification of spore-bearing bacilli, as follows: (A) Cell body of the parent bacillus unaltered in shape (Fig.
[Pg 139] (B) Cell of the parent bacillus altered in shape.
The endo-spores remain within the parent cell for a variable time (in one case it is stated that germination of the spore occurs within the interior of the parent cell-"endo-germination"), but are eventually set free, as a result of the swelling up and solution of the cell membrane of the parent bacillus in the surrounding liquid, or of the rupture of that membrane.
At this stage one of three processes (but the particular process is always constant for the same species) may be observed: (a) The spore grows out into the new bacillus without discarding the spore membrane (which in this case now becomes the cell membrane); e.
In those cases where the spore membrane is discarded the cell membrane of the new bacillus may either be formed from- (a) The inner layer of the spore membrane, which has undergone a preliminary splitting into parietal and visceral layers; e.
The new bacillus now increases in size, elongates, and takes on a vegetative growth-i.
- This modification of Dorset's egg medium (quod vide page 174) is preferred by some for the growth of the tubercle bacillus of the human type.
For example, the original mixed cultivation when examined microscopically was found to contain a Gram positive micrococcus, a Gram positive straight bacillus and a Gram negative short bacillus.
will inhibit the growth of practically everything but the Bacillus coli communis.
A mixture containing the Bacillus typhosus and the Bacillus aquatilis sulcatus, for example, may be planted on two slanted agar tubes, the one incubated at 40°C.
After twenty-four hours' incubation the first will show a pure cultivation of the[Pg 256] Bacillus typhosus, whilst the second will be an almost pure culture of the Bacillus aquatilis.
, and Bacillus mesentericus vulgatus, thermal death-point 60°C.
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