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Example Sentences for "backcrossed "


Molecular cloning Selected mutants were backcrossed until the temperature sensitive phenotype segregated 2:2 and was shown to be linked to the GFP mislocalization phenotype.

[ 4 ] , we consider a backcross experimental design originating from a cross of two homozygous inbred lines, differing in the trait of interest, and producing heterozygous lines that are backcrossed to one of the initial homozygous parental lines.

10 TCR transgenic mice specific for ovalbumin peptide residues 323-339 (OVA323-339) and restricted by I-A d [ 20 ] were backcrossed onto BALB/cJ background (>16 generations).

Tag-TCR1 (referred to hereafter as TCR) transgenic mice expressing a T cell receptor transgene with specificity to Tag were generated in the B6D2F1 background and backcrossed to C3HeBFe for 20 generations [ 28 ] .

These IL-10 -/-mice were backcrossed to DBA/1 for six generations and further backcrossed for an additional two generations to DBA/1 (Jackson Laboratories, Bar Harbor, MA, USA) in our laboratory.

Strains BXHD1, BXHE1 and BXHE2 were backcrossed to C57BL/6J for one generation before sibmatings were begun.

Although the chimeric mice are usually backcrossed to B6 to dilute the contribution of the 129 genome, the knockout strains are always a mixture of the two genomes (Figure 1).


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