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Sentences for "bacterially "
15 mg/ml creatine kinase), reaction buffer (4 mM magnesium acetate, 1 mM DTT), 500 nM bacterially expressed 6xHis-UBCs, 100 nM 6xHis-tagged human E1, and 0.
Upon affinity purification on Ni-NTA resin, HA-Pop1p/His-Myc-Pop2p complexes were incubated with bacterially expressed, phosphorylated Rum1p.
Although the crystal structure of the SKP1-SKP2 complex, derived from bacterially expressed proteins, revealed a single SKP2 monomer bound to SKP1 [ 28 ] , biochemical studies showed that budding yeast Cdc4p, a close homologue of Pop1p\Pop2p, forms homooligomers when expressed in insect cells (Correll and Deshaies, personal communication).
Antibodies Rabbit antisera were raised at Josman LCC (Napa, CA) against bacterially expressed MBP-Psh1p, GST-Pcu1p, GST-Pip1p, and GST-Pop1p.
15 mg/ml creatine kinase), reaction buffer (4 mM magnesium acetate, 1mM DTT), 500 nM bacterially expressed 6 × His-UBCs, 100 nM 6 × His-tagged human E1, 0.
Monoclonal antibody NMT-1 and polyclonal antibodies NMT-4 and NMT-5 also detect bacterially expressed partially purified nmt55/p54 nrbprotein (results not shown).
Recombinant Hh protein The Hh protein used in the studies described here was bacterially overexpressed amino-terminal human Shh modified at its amino-terminal cysteine by an octyl maleimide moiety [ 21 ] .
Results Agonist Dependent ERß Interactions with N-CoR and SMRT To investigate ERß interactions with corepressors, we examined the interactions of full length ERß (amino acids 1-530) with bacterially expressed C-terminal NR interacting domain of N-CoR (amino acids 1944-2453) in vitro (Fig.
The purified DNA template was bacterially subcloned followed by plasmid isolation and sequencing.
Each sample was tested by immunoblot against Jurkat cell lysates with a 1:100 dilution of sera, and by EIA against a bacterially produced U1-70 kD fusion protein that comprised residues 1-205 of u1-70 kD.
Detailed comparisons of the response to wild-type and actA mutant infections were designed to uncover gene-expression programs specific to the motility phase of the infection, when bacterially directed actin polymerization is induced to move the bacteria through the cytoplasm.
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