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Discussion Spiegelman and collaborators [ 34 35 ] asked bacteriophage Qß only to "go forth and replicate" in the medium containing Qß replicase, and numerous functions were lost.

Treatment of agarose slices linearized the plasmid DNA and allowed for plasmid size determination using a concatomerized bacteriophage lambda standard (New England BioLabs, Beverly, MA) (5).

These bacterial proteins form a small family that is additionally represented in Salmonella paratyphi A, the temperate bacteriophage u136 of Lactococcus lactis (ORF252-encoded protein) and a Shiga toxin-converting phage from E.

The poorly characterized phage-or prophage-specific genes that are frequently observed in these neighborhoods include ORF15 ( Streptococcus thermophilus bacteriophage 7201), ORF86, ORF100a ( Staphylococcus aureus temperate phage ?

SLT) and ORF364 (bacteriophage ?

31.

The alternative explanation, namely that the gene coding for the common ancestor of the Redß and RecT already co-occurred with the sbcC gene is far less likely because it would require over 10 independent losses of this, apparently, functionally advantageous organization in different bacterial and bacteriophage lineages.

Several bacteriophage and transposons were identified in the S.

coli K-12 strains, plasmids, and the bacteriophage used in this study are listed in Table 1and Figure 1.

The use of bacteriophage for the treatment of bacterial infections is an old idea [ 4 ] that not only caught the imagination of at least one novelist [ 5 ] it was practiced with sporadic successes worldwide in the 1920s and 1930s.

Not only do bacteria become increasingly refractory to the majority of antibiotics as their rate of growth declines, but they also adsorb to and replicate bacteriophage less efficiently [ 24 33 ] , as is evident from the PRA values in Fig.

Prospects for phage therapy This is a methodological study to develop and experimentally evaluate procedures to measure the efficacy of antibacterial treatment and to screen bacteriophage for their therapeutic potential.

For example, the kinetics of inactivation of a bacteriophage by potassium ferrate was studied with the F-specific RNA-coliphage Q beta.

txt Introduction The RNA polymerase from bacteriophage T7 has a relatively narrow specificity for a particular promoter sequence, making it an extremely useful tool for molecular biology and biotechnology applications.

This mutation resembles the bacteriophage T3 promoter.

The structural and kinetic properties of the Group I self-splicing intron from the thymidylate synthase ( td ) gene in bacteriophage T4 have been extensively studied.

Materials and Methods Gene targeting Bacteriophage clones containing portions of the ?

1 gene were isolated from a mouse 129Sv genomic library (Stratagene, La Jolla, CA
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