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Sentences for "bam "
Bif and bam were early favorites, followed by an off! Then newer impacts came along, pow and kazowie , and in this age of electronic armaments, whoosh and zap have replaced the standard artillery bang bang and rat-a-tat-tat .
To prepare hexa-histidine amino terminally-tagged RGS3T, we modified RGS3T cDNA [ 24 ] using PCR with primers containing restriction sites (the 5' primer incorporated a Bam HI site, whereas the 3' primer bore an Nde 1 site).
The PCR product was digested with Eco RI and Bam HI and ligated into the Eco RI and Bam HI restriction sites of pGBT9 and pGAD424 to produce constructs pGBT9/proHB-EGFEx and pGAD424/proHB-EGFEx, respectively.
The PCR product was digested with Eco RI and Bam HI and ligated into the Eco RI and Bam HI restriction sites of pGBT9 to produce construct pGBT9/prodelHB-EGF.
The PCR product was digested with Eco RI and Bam HI and ligated into the Eco RI and Bam HI restriction sites of pGADA424 to produce construct pGAD424/LTBP-3 EGF
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