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Results and discussion Fifty six (56) different RNA samples were prepared from different regions of muscle biopsies from 28 individuals (15 Duchenne muscular dystrophy (DMD) patients, 13 normal controls).

Muscle biopsies from five 4-6 yr old DMD patients, and five 10-12 yr old patients were selected, each biopsy split into two parts, and RNA isolated independently from each of the 20 biopsy fragments.

This method of data interpretation showed that some muscle biopsies showed very little variance between different regions of the same biopsy, while other patient biopsies showed considerable variability (see Fig.

One was a second set of five normal male biopsies ages 5-12 yrs (controls 2a, 2b), and the third control set was three normal age-matched female biopsies ages 4-13 yrs (controls 3a, 3b) (Fig.

Mixed samples from different regions of the same biopsies showed the same, or only slightly more variation (mixed controls c1, c2, and c3, mixed DMD 6-9 yrs).

As described in the text, all biopsies had two different regions of the same biopsy expression profiled separately.

At day 4 reepithelialization and granulation tissue growth had begun as shown in the 6 mm biopsies in figure 1B.

Northern analysis of wound biopsies showed that production of type I collagen and fibronectin by the null mice, at the beginning of wound healing, was lower than that by the wild-type mice.

RNA analysis Circular, full-thickness dorsal cutaneous biopsies of 6 mm diameter were made on the backs of anesthetized mice and wound sites, including the uninjured epithelial margins, were excised 2, 4, and 6 days post wounding as described above.

However, core needle biopsies of breast cancers, for example, weigh in the 10-25 mg range and yield only 3-15 µg of total RNA.

RNA Preparation and reverse transcription Total RNA was extracted from the frozen mouse skin biopsies of 30 animals as described by Chomczynski and Sacchi (1987) [ 32 ] .

Trained medical record abstractors from the NMTR also collected data from area laboratories on all benign prostate biopsies during the same time period.

bathed ileal biopsies from CF patients in taurocholate solutions.

54 more results not shown.
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