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Example Sentences for "biosciences "


Qualitative PCR A panel containing human total RNA from 20 different tissues was purchased from Clontech (BD Biosciences Clontech).

Primers and positive control for the glyceraldehyde-3-phosphate dehydrogenase housekeeping gene were from Clontech (BD Biosciences Clontech, Palo Alto, CA).

Membranes were then washed the following day 3 times for 5 min in Buffer B with gentle shaking, then incubated for 1 h with gentle shaking at room temperature with horseradish peroxidase (HRP)-conjugated anti-rabbit immunoglobulin G (Amersham Biosciences Corp.

We used the tumor tissue micro-array technique using the high-density lung tissue arrays from Clinomic Biosciences Inc, MA.

Cytokine Bead Array We studied the expression of circulating levels of cytokines IFN-?

, TNF-?

, IL-10, IL-5, IL-4 and IL-2 in sera of patients using a new cytokine bead array system (BD Biosciences San Diego CA, USA).

Clones were selected at random and used as ?

32P-dCTP (Amersham Biosciences Corp, Piscataway, NY, USA) probes on dot blots or Southern blots containing amplicon DNA from the original normal and metastatic cells to verify whether these sequences are actually present in the normal cell DNA sample and missing in the metastatic cell DNA samples.

These samples were used by Radius BioSciences to print 84 12 cm × 8 cm filters.

Sloan's ultimate goal is to make science careers more attractive to talented young people like Herzer, a 2003 PSM graduate in Applied Biosciences from the University of Arizona, who believes his PSM makes him more marketable to science-based businesses.

Brigham, coordinator of the Applied Biosciences PSM program at the University of Arizona.

Antibodies The following monoclonal antibodies were purchased from BD Biosciences Pharmingen (San Diego, California, United States): FITC-, Cy5-, and PE-conjugated anti-CD4, -CD8, -CD3, -CD14, -CD69, -CXCR3, -CCR3, -CCR4, and -CCR5.

Cells were harvested, fixed with formaldehyde, permeabilized with saponin, and intracellularly labeled for IFN-?

and IL-4, in addition to staining for surface CD69, CD4, and CD8 according to the manufacturer's recommendations (Fastimmune, BD Biosciences Pharmingen).

Standard antibody-based enzyme-linked immunosorbent assay was used to measure supernatant concentrations of IP-10, MIG, IL-4, and IFN-?

according to the manufacturer's instructions (R&D Systems and BD Biosciences Pharmingen).

Monoclonal antibodies VCAM, STRO-1, ICAM-1(CD54), CD105, CD29, and MF20 were from Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, Iowa, United States), CD73, CD44, and ALCAM(CD166) were from BD Biosciences Pharmingen (San Diego, California, United States).


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