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Sentences for "biotin "
A total of 8 µg each of H9 (control sample) and H9/Tat (test sample) mRNAs were reverse transcribed into Biotin and Dinitrophenyl (DNP) labeled cDNA, respectively.
Two rounds of amplification (reaction time: 10 minutes) with the tyramide biotin system (NEN) were used to detect E2F.
Insect cell extracts were passed over a column consisting of a double stranded DNA containing two high affinity NFAT sites immobilized on streptavidin sepharose via a biotin at one 5' end.
Secondary biotin anti-IgG antibody (guinea pig IgG Fc specific, Jackson ImmunoResearch, West Grove, PA) was diluted 1:10,000 in 1%BSA/PBS.
The biotin moiety at the N-terminus was separated from the peptide sequence of interest by a 4 amino acid spacer.
05% Tween 20, and biotin labeled anti-mouse IgG (100 µl/well, 125 ng/ml diluted in PBS containing 1% dry fat-free milk) was added.
05% Tween 20 and biotin labeled anti-mouse IgG (100 µl/well, 125 ng/ml diluted in PBS containing 1% dry fat-free milk) was added.
Although the abnormal carnitine ester present in the proband is elevated in organic acidemias and is not usually present in mitochondrial cytopathies, we speculate that due to compromised ATP synthesis, secondary alterations may be generated in these patients by changes in mitochondrial protein synthesis, decrease in Krebs cycle intermediates and increased free radical formation, that could be responsible for a secondary impairment of mitochondrial biotin dependent carboxylases, but further elucidation is necessary before we could arrive to a conclusion in this regard.
Unbound streptavidin is then blocked with 50 µl of 2 mM biotin at room temperature for 1 hr.
5 µl 10 mM Biotin 11-UTP and 4.
We previously showed that biotin moieties can be attached covalently to the outer surface of adenoviral vectors without appreciable effect on their infectivity under carefully controlled biotinylation conditions [ 10 11 ] .
CMV-LacZ was performed by using sulfo-NHS-LC-biotin (Pierce Chemical, Rockford, IL), a biotin derivative containing an N -hydroxysuccinimidyl ester (NHS) that reacts with primary amino groups.
CMV-LacZ on the surface of streptavidin-coated wells is not attributable to the absence of accessible biotin moieties on the surfaces of a fraction of viral particles or the presence of unreacted biotinylation reagent in the biotinylated Ad5.
To the biotin moieties on the solid surface, biotinylated adenov ... by using avidin as a molecular bridge between the biotin moieties on the solid surface and the viral surface.
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