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” Now recall the concept of a reaction graph, a bipartite graph with nodes representing chemical species and lines called hyperedges leading from each set of substrates to a box and from the box to the product species of that particular reaction.

” In fact, a technology graph is deeply similar to a chemical-reaction bipartite graph from a founder set of organic molecules, where the molecules are the objects and the reaction hyperedges linking substrate and products are the transformations among the objects.

A novel RING finger bridged bipartite nuclear localization signal is responsible for mammalian COP1 nuclear localization In a typical bipartite NLS, the distance between the two clusters of positively charged amino acids is usually around 10 amino acids.

Both clusters of positively charged amino acids are required and can function as a classic bipartite NLS when placed within the right distance (Fig 7).

A RING finger sandwiched between a bipartite NLS could provide additional regulatory mechanisms for nuclear import.

We have defined MmCOP1 NLS as a RING finger mediated bipartite NLS variant, which is composed of 96 amino acids surrounding the RING finger domain (Fig 8).

In contrast, the AtCOP1 NLS has been identified as a classic bipartite nuclear localization signal, consisting of 21 amino acids in the intermediate region of coiled-coil domain and the WD-40 domain [ [ 17 ] , Fig 8].

falciparum PP5 is primarily nuclear [ 9 ] , although it is not well conserved in the relevant C-terminal region but contains a potential bipartite nuclear localization signal not found in other PP5 sequences reported thus far.

The extended imprinted region appears to have a bipartite structure in that it contains at least two separate imprinted sub-domains.

The TOX protein is 526 amino acids with an acidic N-terminal domain, a bipartite nuclear localization signal sequence and a single centrally located HMG-box motif [ 15 ] .

This sequence is the likely nuclear localization signal (NLS) for these proteins [ 15 ] , similar to the bipartite NLS found in nucleoplasmin [ 36 ] .

The common distal breakpoint is termed BP3 and has a bipartite structure [ 15 16 ] .

The resulting bipartite transcript would yield a PCR product of 93 bps.


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