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Sentences for "blastocyst "
No embryos developed to the blastocyst stage when nocodazole-treated cells were used as donors regardless of the maturation stage of the recipient oocytes.
To our knowledge, this is the first report that describes blastocyst formation of embryos reconstituted with M I oocytes in mammals.
Moreover, some reconstructed embryos produced by transferring confluent cells developed to the blastocyst stage.
The blastocyst formation rate of reconstructed embryos produced by transferring confluent cells into M I oocytes was significantly lower than that of embryos reconstituted with confluent cells and M II oocytes although there were no significant differences in percentages of embryos with swollen nuclei between different maturation stages of recipient oocytes.
In contrast, no reconstructed embryos developed to the blastocyst stage regardless of the maturation stage of the recipient oocytes when nocodazole-treated cells were transferred, although nuclear swelling was observed in 34.
In the bovine, the potential of reconstructed embryos with or without the polar body to develop to the blastocyst stage is not different [ 14].
6% of reconstructed embryos produced by transferring confluent adult somatic cells into enucleated M II oocytes matured in the protein-free medium developed to the blastocyst stage.
The blastocyst formation rate in our study is similar to results from the recent reports in which porcine adult somatic (cumulus) cells (5-10%) [ 29, 51] or fetal fibroblasts (1-16%) [ 11, 29, 51, 52, 53, 54, 55, 56, 57] were transferred into enucleated M II oocytes matured in media supplemented with pFF.
The embryos were examined for cleavage and blastocyst formation at 2 and 7 days after culture, respectively.
txt Introduction Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of the blastocyst that can be maintained in culture for an extended period of time without losing differentiation potential.
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