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Example Sentences for "blastocysts "


Transgenic mice and embryos can be generated from these ES cells by standard injection into blastocysts and subsequent breeding of the resulting chimeric mice.

Aggregates that formed blastocysts after overnight culture were allowed to continue their development in utero by transferring them to a pseudopregnant surrogate mother.

There were no significant differences in mean numbers of cells in the blastocysts between different maturation stages of the recipient oocytes.

Discussion The results of the present study demonstrated the in vitro developmental ability to blastocysts of porcine embryos reconstituted with somatic cells and enucleated M I oocytes.

In addition, quality of the blastocysts based on the mean number of cells (45.

Conclusions We have shown that porcine M I oocytes have a potential to develop into blastocysts after nuclear transfer of somatic cells.

At the end of the culture period, the number of cells in blastocysts was examined.

The blastocysts were placed on slides with a drop of mounting medium consisting of glycerol and PBS (9:1) containing 0.

A cover slip was placed on the top of the blastocysts and the edge was sealed with fingernail polish.

The numbers of cells in blastocysts were analyzed using Student t-test.

ES cell:embryo aggregates were cultured overnight and those that formed blastocysts were transferred to the uteri of psuedo-pregnant females surrogate mothers.

Targeted ES cell clones were injected into C57Bl/6J blastocysts and chimeras obtained.

After multiple trials (and errors), ES cell lines from the 129 strain and blastocysts from the C57BL/6 (B6) strain have shown themselves to be superior in terms of efficiency and reliability.


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