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Sentences for "bleb "
, LDH release, is associated with dramatic bleb dilation.
From time 28 to 38 minutes, the second phase of dye uptake is observed, and this phase is associated with large bleb dilation (Fig 6, red arrows).
These results demonstrate that COP activity is associated with initial bleb formation, whereas LDH release (i.
, cytolysis) is associated with dramatic bleb dilation.
movie 1 To better appreciate the dynamic nature of bleb formation and to clearly observe the rapid nuclear staining associated with bleb dilation, we created time-lapsed movies in which the bright-field and fluorescence images were merged into a single video (Fig 2, EB.
, auto replay), and by focusing during each sequence on a single cell within the field of view, it can be seen that fluorescence increases slowly during bleb formation, but that intense rapid dye staining occurs during the bleb dilation phase.
Thus, the structural element(s) that are required for localized evagination of membrane appears to be membrane-associated and/or "pulled" from the cytoplasm during the initial bleb formation.
Furthermore, activation of COP provides the initial driving force for osmotic swelling and bleb formation.
LDH release, indicative of the final phase of MTX-induced cell death, is associated with massive bleb dilation.
Using time-lapse videomicroscopy, we have shown that MTX-induced release of LDH from vascular endothelial cells is associated with massive bleb dilation and rapid staining of the nucleus with vital dyes [ 3 ] .
Zeiosis, which comes from the Greek word Z?
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meaning "to boil over" [ 21 ] , is characterized by violent cytokinesis with continuous bleb extension and retraction.
U73343 alters MTX-induced bleb formation.
The dynamic nature of the MTX-induced bleb formation, bleb dilation, and ethidium uptake can be observed by viewing the time-lapse movie associated with this experiment (see Additional File 1).
7 more results not shown.
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