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Sentences for "blebs "
Although MP-release was not investigated, authors showed micrographs of stimulated cells with membrane blebs of about 3 µm in diameter.
An example of MTX-induced membrane blebs is shown in the micrograph in Fig 5which was obtained with Hoffman optics and simultaneous bright-field and fluorescence illumination.
After 50 minutes of MTX treatment at room temperature, YO-PRO staining is prominent in the nucleus as expected, but is also visible in giant membrane blebs seen surrounding each cell in the field of view.
However, blebbing presumably requires a change in osmotic pressure within the cell and so we reasoned that the blebs may be associated with the second phase of vital dye uptake.
Interestingly, the videos show several examples of blebs forming on the surface of pre-existing blebs during the dilation phase.
In isolated bovine aortic endothelial cells (BAECs), the opening or activation of COP is associated with formation of spherical membrane blebs with a diameter of 3-5 microns [ 3 ] .
, 20 min after addition of MTX) large membrane blebs were observed on essentially all the cells within the field of view (Fig 5A, see examples indicated by blue arrows ).
As seen in Fig 5B, essentially no blebs are observed at 25 min (i.
We previously showed that MTX causes the appearance of membrane blebs on the surface of the endothelial cells [ 3 ] .
Initially these blebs are of small diameter (3-5 microns) and bleb formation correlates with the first phase of vital dye uptake.
However, during MTX-induced cytolysis, as indicated by the release of LDH, the membrane blebs actually exhibit a dramatic increase in diameter.
In static images, the morphological changes associated with apoptosis appear as membrane blebs covering the surface of the cells [ 31 32 33 ] .
However, time-lapse images reveal that blebs protrude and retract in a dynamic fashion, a hallmark of zeiosis [ 22 23 ] .
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