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Sentences for "blots "
" The writing is small and clear, with the upstrokes and downstrokes rather long in comparison with the level letters; but some small blurs and blots show that the letter was written in unusual haste.
But the blots were more expressive to me than the best composition; for they showed me that Peggotty had been crying all over the paper, and what could I have desired more?
Preliminary results using western blots supports the idea that genes which were altered in H9/Tat system also showed a similar level of change in few of the tested genes (Figure 3C).
Northern blots Total cellular RNA was extracted using the RNAzol reagent (Gibco/BRL).
These observations strongly suggest that results for significantly altered genes should be confirmed with other traditional techniques such as Northern blots or RNase protection assays prior to reporting the fold increase.
Similarly an anti-apoptotic gene A20 is also over-expressed in leukemic LGL, but protein expression was absent when Western blots were performed with monoclonal antibodies raised against the amino acid sequence derived from A20 (data not shown).
To our surprise, none of the three down-regulated genes studied exhibited differential expression in Northern blots when the cDNA fragments of these genes were used as probes.
Hybridization and washing of the blots were performed as described by Engler-Blum et al [ 15 ] .
All blots were developed with the ECL plus reagent and exposed to Hyperfilm.
Tag expression at zero and late cell densities Figure 8Ashows the correlation plot for the objective, "expression potential" intercept measurement at zero cell density (defined above) and Tag measured from Western blots in Figure 2.
Probing the same blots for Coll III revealed no cCAF-induced difference in the level of production of this protein (Fig.
In vivo, Coll I was also slightly increased by treatment with the chemokine at 3 days after wounding but did not increase to higher levels than the control at 7 days, whereas the same blots probed for Coll III showed similar levels in treated and control at both 3 and 7 days (Figure 6C).
Western blots were developed using ECL+ (Amersham Pharmacia).
Western blots were performed using total serum at a dilution of 1/2000 and chemiluminscent detection.
The colony blots were then wrapped in saran wrap and exposed to X-ray film at room temperature for 24 h at -80°C.
130 more results not shown.
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