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(See Boney M's seminal 1979 disco hit, which runs, "Ra-ra-rasputEEN , lover of the Russian queen.
The supercritical carbon dioxide (SCCO 2 ) extracts of Idaho red sand (Figure 1) and a Lake Boney Basin Antarctica soil, which contained relatively few bacteria (Table 1), showed detectable signature molecules in the extracted samples using either a diode array detector or an amperometric detector (Figure 1).
To confirm that soil samples yielding negative results and those yielding signatures of redox moieties were different because of the presence and absence of biological entities, we counted viable organisms and extracted DNA from the heated control sand, a Beacon Valley Antarctic soil, a Lake Boney Basin Antarctic soil, and the Idaho red sand (Table 1).
, the Idaho red sand and Lake Boney Basin soil) also contained both DNA and cultivable bacteria.
Figure 5shows results of XTT reduction by the control and the life-positive Boney Basin, Antarctica soil.
We easily detected a signature of biological electron transport within a biologically poor, sandy soil containing about 30,000 cultivable cells per gram (Idaho red sand), and also from a soil from the extreme environment of Lake Boney Basin, Antarctica (Table 1).
Though the Boney Basin soil readily reduced XTT, it only very slowly reduced DCIP (data not shown), and the rate of reduction was not significantly different between untreated soil and killed controls.
Though the amount of DNA observed in the Lake Boney Basin soil was actually higher than seen in the Idaho red sand, the microbial counts were lower (Table 1).
Other extraterrestrial surrogate soils were collected from Beacon Valley and Lake Boney Basin, Antarctica.
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