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Sentences for "broth "
- If it is desired to sterilise gases before admission to a vessel containing a pure cultivation of a micro-organism, as, for instance, when forcing a current of oxygen over or through a broth cultivation of the diphtheria bacillus, this can be readily effected as follows:[Pg 41] 1.
Filter the supernatant broth through Swedish filter paper.
Weigh out rice powder, 100 grammes, and rub it up in a mortar with the milk and broth mixture.
Weigh out rice powder, 100 grammes, and rub it up in a mortar with the milk and broth mixture.
Prepare first and second dilutions of the inoculum as directed for plate cultivations (vide pages 228-229, sections 4 to 6), substituting tubes of nutrient broth for the liquefied gelatine.
Remove a drop of the broth from the second dilution tube with a large platinum loop; raise the bell glass and deposit the drop on the centre of the cover-slip.
deep, of broth cultivation of a vigourous aerobe-e.
Inoculate an agar slope and a broth tube from the emulsion in the stock dish corresponding to each of these specially selected numbers.
-Plant the organism in broth and incubate under optimum conditions.
Transfer the emulsion to a tube of sterile nutrient broth and incubate.
Prepare cultivations in nutrient broth and incubate under optimum conditions.
Prepare cultivations in nutrient broth and incubate under optimum conditions for forty-eight hours.
Suspend the flask containing the twenty-four-hour-old broth culture (step 3) in the water-bath at 60° C.
Fill the sac with nutrient broth and place it, glass tube downward, in a tube containing sufficient sterile nutrient broth to cover the sac to the depth of 1 cm.
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