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Example Sentences for "brucella "


A number of investigators have attempted to devise methods for genotyping Brucella strains.

RFLP-PCR of the omp 2 locus is not constrained by issues of reproducibility and has been useful for the differentiation of Brucella species [ 6 ] and for differentiation among isolates from marine mammal hosts [ 7 ] .

This paper describes the first application of microsatellite fingerprinting to Brucella isolates.

We have employed microsatellite fingerprinting for genotyping Brucella species based on the number of tandem repeats of the DNA sequence "AGGGCAGT" at eight loci in the genome.

The type strains of all the classical Brucella species and biovars were surveyed to assess the discriminating power of this fingerprint technique.

Results Identification and characterization of the tandem repeat loci Multiple tandem repeats of the octameric sequence "AGGGCAGT" were initially discovered when searching sequence files generated from the shotgun sequencing of Brucella abortus biovar 1, strain 9-941 for a subsequence (TAGGGC) of IS 711 .

The repeat regions and approximately 200-bp of upstream and downstream flanking sequence were individually compared to the Brucella melitensis biovar 1 and Brucella suis biovar 1 published genome sequences.

To facilitate comparisons among the three Brucella genomes, Table 1indexes the coordinates of each VNTR locus in the B.

Among the three sequenced Brucella genomes, the number of complete repeat units ranged from one copy (e.

These sequences had a higher than normal level of sequence divergence than is typically found among Brucella species, but a consensus sequence was evident (top line of Fig.

Development of the PCR assay We wanted to examine the depth of variability of the tandem repeat loci among other Brucella species, and more importantly, whether variability also occurred among biovars and among independent isolates within a biovar.

The PCR templates consisted of methanol-fixed bacteria from a broad array of Brucella strains and isolates (listed in Table 3and the Materials and Methods section).

Comparison of alleles among the BrucellaReference Strains for all species and biovars A panel of 19 pure Brucella cultures, composed of all of the Brucella species and biovar FAO/WHO Reference Strains, was assayed by the HOOF-Print procedure.

19 more results not shown.
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