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Sentences for "buffered "
The cells were then washed with phosphate buffered saline (PBS) and then incubated in medium (Ham's F12 or Dulbecco's modified Eagle's medium (DMEM)) containing 100 µM bile acids for 1 to 5 h as detailed in the Figure legends.
Beginning in 1995, a series of papers reported the use of 13C-NMR to study the ionization of a close analogue of bilirubin-IX?
, mesobilirubin-XIII?
(MBR), along with several soluble reference acids, in buffered mixtures of (C 2H 3 ) 2 SO and water [ 3 4 5 6 7 ] .
44 obtained by solvent partition between chloroform and buffered aqueous solutions [ 2 ] .
EYFP-cPLA 2 /ECFP-cPLA 2 S505A- or pECFP-cPLA 2 S727A-transfected MDCK cells grown on MatTek plates were quiesced overnight in serum-free medium, washed with and incubated in Hank's balanced salt solution (HBSS) additionally buffered with 25 mM HEPES pH 7.
Abbreviations aa, amino acid, Ab, antibody, bp, base pair, CaPO 4 , calcium phosphate, C/EBPß, CCAAT-enhancer binding protein-beta, CAT, chloramphenicol acetyl transferase, DMEM, Dulbecco's Modified Eagle Medium, GST, glutathione-S-transferase, MAPK, mitogen activated protein kinase, PBS, phosphate buffered saline, PMSF, phenylmethylsulfonyl fluoride, SRE, serum response element, SRF, serum response factor, TCF, ternary complex factor.
Skin was removed, washed with phosphate buffered saline (PBS), and incubated overnight in 0.
Abbreviations DMEM, Dulbecco's modified Eagle's medium, ECM, extracellular matrix, FBS, fetal bovine serum, GAPDH: glyceraldehyde-3-phosphate dehydrogenase, PBS, phosphate buffered saline, SPARC, secreted protein acidic and rich in cysteine, also known as osteonectin and BM-40, SD, standard deviation.
, Bedford, MA) which were then incubated for 2 h in a blocking solution containing 5% non-fat dry milk in phosphate buffered saline containing 0.
For light microscopy, specimens were either fixed in 10% neutral buffered formalin, decalcified and embedded in paraffin or processed non-decalcified and embedded in methyl methacrylate.
All buffered solutions and other reagents for organic synthesis were analytical grade and freshly prepared.
Spectrofluorimetric analyses Stock solutions of OxyBURST Green H 2 HFF BSA were prepared in phosphate buffered saline (PBS) and stored under nitrogen at 4°C.
Briefly, cryostat sections were fixed with acetone, dried, and rinsed in phosphate buffered saline (PBS).
Following color development, the embryos were treated with 2 N NaOH for 1 hr, washed with PBSTx (phosphate buffered saline with 0.
Embryos were dissected in cold phosphate buffered saline (PBS) and staged according to Theiler [ 68 ] .
In most staining experiments pupae were fixed in phosphate buffered saline supplemented with 4% paraformaldehyde.
80 more results not shown.
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