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Sentences for "bullfrog "
Myo1c belongs to the myosin-I class, which contains eight members in humans [ 8 ] and mice [ 9 ] , the bullfrog genome possesses at least two members [ 10 ] .
Results Sequence analysis of IQ domains Examination of the primary sequence of the bullfrog Myo1c neck region reveals an exact repeat of five amino acids located both in the IQ3 region (YRNQP, residues 761-765) and at residues 786-790.
Hydrodynamic analysis of full-length Myo1c To determine the stoichiometry and Ca 2+-dependent regulation of calmodulin binding to Myo1c with all four IQ motifs, we co-expressed calmodulin and full-length bullfrog Myo1c in insect cells using baculoviruses and subjected the purified Myo1c-calmodulin complexes (Fig.
This construct, T701-Myo1c, contained amino acids 701-1028 of bullfrog Myo1c, including all four IQ domains, the entire C-terminal tail, and N-terminal purification and epitope tags (Fig.
Methods Peptide - calmodulin interaction on plates Bullfrog Myo1c IQ peptides were synthesized (Genemed Synthesis, South San Francisco, CA) with N-terminal cysteine residues: IQ1 (residues 698-720), CRKHSIATFLQARWRGYHQRQKFL, IQ2 (721-743), CHMKHSAVEIQSWWRGTIGRRKAA, IQ3 (744-766), CKRKWAVDVVRRFIKGFIYRNQPR, and IQ4 (767-791, native cysteine at residue 767), CTENEYFLDYIRYSFLMTLYRNQPK.
Baculovirus constructs Using methods described previously for rat Myo1c [ 43 ] , we cloned full-length bullfrog Myo1c into the baculovirus transfer vector pBlueBacHis2B (Invitrogen, Carlsbad, CA), introducing an N-terminal hexahistidine tag for purification and a DLYDDDDK epitope tag for antibody detection.
Protein expression and purification Bullfrog Myo1c or its neck-tail fragment (Fig.
Xenopus calmodulin is identical to all other sequenced vertebrate calmodulins, including bovine calmodulin [ 46 ] , we presume that bullfrog calmodulin is also identical.
The principal neurons of bullfrog sympathetic ganglia express a SPR that has been well characterized with regard to its structure and function.
The bullfrog sympathetic ganglia SPR (bfSPR) has 69% homology to the rSPR at the amino acid level.
Perhaps most importantly, nine of the ten residues that have been demonstrated to be important for SP binding to mammalian receptors are conserved in the bullfrog receptor.
Though residues 1-21 of the mouse SPR are primarily conserved in the rSPR, only 5 of the 21 residues are conserved in the bullfrog SPR.
Electrophysiology Single neurons were dissociated from bullfrog sympathetic ganglia as described previously [ 19].
Our aim in creating Bullfrog and Spot is to provide simple tools that enable researchers at all levels to analyze their data in multiple ways without having to use more complex software, without having to call in bioinformatics experts, and without having to learn to program in scripting or database languages.
Manipulating data sets in Bullfrog Bullfrog and Spot can be used to select genes (probes, probe sets or spots) that behave in specific ways across multiple experiments by using a combination of more than 20 different qualitative and quantitative criteria (Figure 1).
18 more results not shown.
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