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Sentences for "calmodulins "
Purified Myo1c apparently includes 2-3 calmodulins per Myo1c heavy chain [ 19 20 21 ] , calmodulin su ... ion can increase the stoichiometry to as many as 4 calmodulins per Myo1c [ 21 ] .
Ca 2+dissociates one or more calmodulins from the myosin-calmodulin complex, which apparently elevates ATPase activity and inhibits motility [ 11 ] .
731 for three calmodulins per Myo1c complex) that the precise value did not significantly affect the final molecular-mass value.
Full-length Myo1c bound ~3 calmodulins per Myo1c at 4°C in the presence of EGTA or CaCl 2 (Table 3).
One of the bound calmodulins was only weakly associated, as elevation of the temperature to 25°C induced the release of 1 mole of calmodulin in the presence of EGTA.
3 mole of calmodulin per mole of Myo1c, reinforcing the suggestion that Ca 2+induced the dissociation of most calmodulins at 25°C and that this loss of light chains resulted in aggregation.
Sedimentation at 25°C in EGTA gradients supplemented with 5 µM calmodulin resulted in the retention of ~3 calmodulins per T701-Myo1c.
2 calmodulins per T701-Myo1c (mean ± standard error) in six experiments, three separate preparations analyzed in duplicate.
1 bound calmodulins determined independently by hydrodynamic analysis (Table 4).
Nevertheless, our approach was validated by the demonstration that the number of calmodulins per T701-Myo1c was identical in hydrodynamic and gel-scanning experiments, at least in EGTA at 4°C.
5, Table 2), Ca 2+decreased the number of calmodulins bound to T701-Myo1c at high ionic strength.
How many calmodulins are bound to Myo1c in the cell at increased Ca 2+concentrations?
We therefore infer that at a physiologically significant temperature and at a cellular ionic strength, Ca 2+triggers release of calmodulins from IQ1 and IQ2 from T701-Myo1c, leaving only IQ3 occupied.
Although these results contrast with those reported for mammalian Myo1c, where only one of three calmodulins is released by Ca 2+ [ 19 38 ] , our T701 construct lacks Myo1c's motor domain.
2 more results not shown.
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