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Example Sentences for "centrifugation "


Cells were harvested 4 hours after induction by centrifugation at 7000 rpm for 10 min and washed with TEP buffer (0.

4) using Potter-Elvehjam homogenizer and the insoluble material was removed by centrifugation at 15,000 rpm for 20 min.

After elution from the gel slice, the labeled single-stranded oligonucleotides were desalted by centrifugation through a Sephadex G10 spin column.

Results Residual UCB in the supernatants after centrifugation Many systems initially appeared optically clear, but well over 90% of initial UCB sedimented on centrifugation of most samples below pH 7.

Our centrifugation, 5 min at 14,000 g , was quite mild, and the short 20-minute period between preparation of UCB-DMSO-water systems and centrifugation severely limited the time-dependent growth to large aggregates.

Glycerol gradient ultra centrifugation Fifty micrograms of the enzyme protein in 100 µL of buffer (50 mM Tris HCl, pH 7.

Cells were diluted in buffer S and pelleted by centrifugation and resuspended in buffer F (18 % Ficoll 400, w/v, 20 mM HEPES (pH = 7.

The cell suspension was homogenized in a Dounce homogenizer using a B-type pestle followed by centrifugation at 3000 rpm for 7 min.

When Ca 2+was elevated to 25 µM at 25°C, however, we could not detect substantial full-length Myo1c in solution after sucrose-gradient centrifugation or gel filtration, suggesting that the protein had aggregated.

For example, despite micromolar K d values for calmodulin-IQ peptide interactions, calmodulin remains bound to Myo1c during long gel-filtration or centrifugation experiments, even at nanomolar Myo1c concentrations (Fig.

Recombinant proteins were partially purified by centrifugation of an Sf9-cell extract and Ni 2+-nitrilotriacetic acid chromatography [ 43 ] , further purification was achieved using gel filtration at 4°C on a 25-ml Superdex 200 HR 10/30 column run at 0.

After centrifugation at 33,000-40,000 rpm in an SW 41 rotor for 15-18 hours at 4°C or 25°C, gradients were fractionated from the bottom into ~30 fractions.

Another group trying to repeat the observations of increased levels of LPA associated with ovarian cancer concluded that there was no diagnostic value in the assay, and attributed the discrepant findings as possibly related to different sample centrifugation protocols used by the two groups to remove platelets from the samples prior to analysis [ 20 ] .

Cells were harvested by centrifugation and washed once with phosphate-buffered saline (PBS) containing the following protease inhibitors: 2 mg/ml aprotinin, 2 mg/ml leupeptin, 2 mM phenylmethylsulfonyl fluoride (PMSF), and 0.

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