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English words and Examples of Usage
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Sentences for "centrifuged "
A 5-35% discontinuous sucrose gradient was formed above (1 ml of 5% sucrose: 2 ml of 35% sucrose, both in MBS containing 250 mM sodium carbonate) and centrifuged at 45 000 rpm for 16-18 hrs in SW 55 Ti rotor (Beckman Instruments, Palo Alto, CA).
Following 10 min incubation on ice, lysed cells were centrifuged for 10 min, 10,000 × g at 4°C.
Solubilized BBMV was centrifuged at 10,000 × g for 10 min and supernatant was concentrated to 2 ml by Amicon YM30 ultrafiltration.
The disrupted cells were centrifuged at 8000 rpm and the pellet was resuspended in 500 µL of fresh TEP buffer.
An additional 1 ml of ammonium acetate was added to the pellet, the homogenization was repeated, the contents were centrifuged and the supernatant was collected.
Media from HIV-1 infected cells were centrifuged to pellet the cells and supernatants were collected, and diluted to 1:100 to 1:1000 in RPMI 1640 prior to ELISA.
Immediately prior to use, cells were homogenized on ice in PBS using a glass tissue homogenizer then centrifuged at 35 000 × g for 30 minutes in a Beckman JA20 rotor (4°C).
Immediately prior to use, cells were homogenized in PBS using a glass tissue homogenizer then centrifuged at 35 000 × g for 30 minutes in a Beckman JA20 rotor.
of buffer and centrifuged again, showed more sedimentation and a progressive rise in [UCB] as one moved down the column of fluid.
31 DMSO was centrifuged because of partial insolubility [ 8 ] and only the supernatant was used.
8 mL aliquots were transferred to polypropylene tubes and centrifuged for 5 min at 25°C and 14,000 g (Mikroliter centrifuge, Hettich, Tuttlingen, Germany).
The gradients were centrifuged at 48,000 rpm in an SW48 rotor for 22 h at 4°C.
Lysates were centrifuged at 15,000 × g for 15 min, and protein concentration of the supernatant was determined by the bicinchoninic acid method.
The medium was centrifuged at 500 g for 5 min, and the amount of radioactivity in the supernatant was determined by scintillation counting.
The medium was collected 48 hrs after the infection (found to be the optimal harvesting time for wtPAI-1) and then centrifuged for 5 minutes (125 × g).
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