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The second step of the insertion involves an obligatory hydrolysis of ATP that is tightly coupled with the transfer of the chelated Mg 2+to the protoporphyrin ring by BchH [ 49 ] .
In some experiments extracellular calcium was chelated by pre-treating the cells with 3 mM EGTA for 15-min.
Several systematic, qualitative reviews [ 2 8 23 24 ] highlight the clinical benefits of desferrioxamine treatment and, with respect to iron loading, acknowledge its ability to maintain harmless hepatic iron levels in properly chelated patients.
The structures identify a zinc ion chelated to the two histidines and the aspartic residue of the MPN + /JAMM sequence.
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