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Sentences for "chelating "
The Histag RGS3T protein was purified on a Ni 2+resin using a Pharmacia Hi-Trap 1 ml Chelating Column (Pharmacia Biotech, Piscataway, NJ).
However, dramatic reduction of copper in SOD1 by knocking out the copper chaperon for SOD1 (CCS), or by mutating the copper chelating residues, did not affect ALS progression in vivo [ 36 48 ] .
Measurement of the magnitude of iron loading is useful in evaluating the effectiveness of the chelating agent, calibrating patient-specific treatment, and, in clinical research, as a determinant of clinical outcome.
The National Library of Medicine's computerized bibliographic database (Medline 1966 to December 1999) was searched using a combination of the following keywords: thalassemia, serum ferritin, urinary iron excretion, hepatic iron, liver, chelation therapy, iron chelation, iron chelating agents, desferal, desferrioxamine, deferoxamine, L1 oral chelate, deferiprone and 1,2-dimethyl-3-hydroxypyrid-4-one.
No significant difference in the reduction of HIC or the presence of liver fibrosis was detected in this subgroup of patients, leading the authors to conclude that both treatments have a similar chelating effect over a relatively short time period.
For chelating intracellular calcium, the cells were preincubated with 5 µM of 1,2-bis(2-aminophenoxy)ethane- N , N , N ', N '-tetraacetic acid, acetoxymethyl ester (BAPTA-AM) for 15 min.
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