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Sentences for "chelation "
In some experiments, Ca 2+was held at <30 nM by chelation with 100 µM EGTA, in other experiments, we added 25 µM exogenous CaCl 2 in the absence of EGTA.
Antiserum production The catalytic domain of MHCK-C was expressed in bacteria and purified using nickel chelation chromatography as described [ 18 ] .
Milligrams of fusion proteins between thioredoxin and DAZAP1 were prepared and purified on His-Bind metal chelation resins (Novagen, Madison, WI).
Although the details differ from midasin, particularly with respect to the subunit composition of the AAA ring, this chelation reaction provides a structural model suggesting that the function of the midasin M-domain may be to regulate the ATPase activity of the AAA protomers in the pseudo-hexameric ring and thus couple ATP hydrolysis to the binding of a protein ligand at the MIDAS site.
The deletion mutant, ?
TPR-PfPP5, was similarly cloned by PCR using a primer corresponding to the internal sequence, and purified by nickel chelation affinity chromatography.
As a complement to frequent blood transfusions, proper iron chelation therapy further improves the quality of the patient's life and extends survival, impeding iron loading complications.
Liver biopsies are neither a convenient [ 23 ] nor patient-preferred [ 2 19 25 ] means of monitoring efficacy in iron chelation therapy.
In the case of iron chelation therapy for patients with thalassemia major, the addition of any good prospective randomized control trial documenting the impact of treatment on body iron (hepatic and/or myocardial) would contribute measurably to the evaluation of the long-term implications of treatment with deferiprone, compared to desferrioxamine.
These compounds may have played a role in the inhibitory effect observed, probably owing to the chelation of the zinc required for the catalytic activity of venom's haemorrhagic metalloproteinases [ 45 ] .
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