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Sentences for "chemiluminescence "
ß-Actin, moesin, and phosphorylated moesin were detected by immunoblotting and the enhanced chemiluminescence detection system.
Blots were revealed using peroxidase conjugated goat anti-mouse IgG antibody and chemiluminescence with an ECL kit (Amersham).
The secondary antibodies were coupled to horseradish peroxidase, and were detected by chemiluminescence using the SuperSignal™ substrate from Pierce (Rockford, MD).
Subsequently, blots were washed three times as described above, and visualized using an enhanced chemiluminescence detection method (ECL, Amersham).
The membrane was then incubated with anti-rabbit IgG peroxidase conjugates, and proteins were visualized using the chemiluminescence method (Luminol reagent, Santa Cruz Biotechnology) and Kodak BioMax light film (Fisher Scientific).
Enhanced chemiluminescence was performed using standard reagents (Amersham Laboratories, Arlington Heights, IL).
The blots were washed three times in phosphate-buffered saline, and the protein complexes were detected with enhanced chemiluminescence detection reagents according to the manufacturer's protocol (Amersham Pharmacia Biotech, Inc, Piscataway, NJ).
The blot was washed once for 5 minutes in PBS-T, incubated with goat-anti-rabbit conjugated HRP (1:10000 dilution) for 1 hour, washed for 5 minutes in PBS-T and developed with Western Lightning Chemiluminescence Reagent Plus (PerkinElmer Life Sciences, Inc.
The Galacto-Light Plus chemiluminescence reporter assay for ß-galactosidase was from Applied Biosystems, Foster City, CA.
Aliquots (20 µl) of the cell lysates were transferred into wells of 96-well microplates and ß-galactosidase was quantitated using the Galacto-Light Plus System chemiluminescence reporter assay in a Microlight ML 2250 luminometer (Dynatech Laboratories, Inc.
Enhanced chemiluminescence reagents were from Amersham (Piscataway, NJ).
Western Lightning Chemiluminescence Reagent (Perkin Elmer Life Sciences Inc.
Following extensive washing in TBST, bands were visualized by the enhanced chemiluminescence method (Roche Molecular Biochemicals) and quantitated by laser densitometry.
Bands were visualized by the enhanced chemiluminescence method and quantitated by laser densitometry.
Following three washes of 10 min each with TBST, the nitrocellulose was probed with horse-radish peroxidase-conjugated anti-IgG (Promega, Madison, WI) and developed using chemiluminescence detection (Pierce, Rockford, IL) according to the manufacturer's instructions.
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