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English words and Examples of Usage
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Sentences for "chemiluminescent "
The secondary Ab was detected using SuperSignal Chemiluminescent Substrate (Pierce).
Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).
Equal volumes of each sample (cytosolic and cytoskeleton-associated components) were subjected to SDS-PAGE, transferred to a nitrocellulose membrane (Bio-Rad) and immunoblotted with primary antibody to ß-actin and detected using horseradish peroxidase-conjugated secondary antibody (Vector Laboratories) and SuperSignal West Dura (Pierce) as a chemiluminescent substrate.
The bands were visualized using a chemiluminescent detection system (ECL, Amersham Life Science, Arlinton, IL).
Immunoreactive proteins were visualized using a horseradish peroxidase-sensitive ECL chemiluminescent Western blotting (WB) kit (Pierce).
Incubation was then performed for ten minutes at room temperature with CDP-Star chemiluminescent substrate (Boehringer Mannheim, Biochemical Products, Indianapolis IN) diluted 1:100 in substrate buffer.
In addition, hybridization and chemiluminescent immunodetection were also performed using the same protocol for genome blot analysis except for the following.
Assays were performed using the chemiluminescent SEAP assay procedure as described by the manufacturer in the Great EscAPe SEAP Assay system (Clontech).
The chemiluminescent signal was measured with a PharMingen Monolight 3010 luminometer and recorded as Relative Light Units (RLU).
Bound antibodies were detected by a chemiluminescent detection system (ECL, Amersham Pharmacia Biotech, Piscataway, NJ) as recommended by the manufacturer's protocol .
Membranes were incubated and developed according to the Enhanced Chemiluminescent Protocol, according to manufacturer's instructions (Amersham).
The primary antibody was incubated in the 5% non-fat milk with the proteins on the membrane overnight at 4°C, and the protein of interest was visualized by the enhanced chemiluminescent method (ECL).
Bound antibodies were detected by a chemiluminescent detection system (ECL, Amersham Pharmacia Biotech, Piscataway, NJ) as recommended by the manufacturer's protocol.
Cyclin E variants in Cdk2 IP were detected by probing with HE12 primary antibody (PharMingen), followed by goat anti-mouse IgG and IgM (Jackson Immunoresearch) secondary antibody conjugated with horse radish peroxidase, and visualized by a chemiluminescent detection system (ECL, Amersham Pharmacia Biotechnology) and autoradiography as indicated above.
After washing in TBST, the ERß protein was visualized with a chemiluminescent detection system (ECL, Amersham Pharmacia Biotech, Piscataway, NJ) as recommended by the manufacturer, and exposed on Kodak XAR film (Eastman Kodak, Rochester, NY).
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