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In the chicken chorioallantoic membrane (CAM) assay, low concentration of cCAF results in chemotaxis of monocyte/macrophages and lymphocytes and the formation of a granulation-like tissue beneath the chemokine-containing pellet [ 3 ] .

Because tissues treated with cCAF have more FN and TN at the time that these cells are entering the wound and chemokines are well known for their chemoattractant properties [ 34 35 ] , we tested whether cCAF stimulates chemotaxis of fibroblasts using transwell assays.

Control for chemotaxis was performed by adding 1000 ng/ml cCAF to both wells of the Transwell unit.

discoideum cells in suspension as well as for efficient chemotaxis and morphogenetic changes in shape during development) [ 1 2 3 ] .

They may represent a unique paracrine mechanism, so called "targeted delivery," by which certain growth factor (vesicle content) is delivered by chemotaxis to certain type/stage specific target cells expressing receptor for Thy-1 ligand.

Mounds of about 10 5cells form as cells stream together though chemotaxis in response to cAMP pulses.

At convergence, this search detected, in addition to the soluble guanylyl cyclases from the animals, several bacterial proteins, including certain methyl-accepting chemotaxis receptors from bacteria such as Desulfovibrio and Clostridium .

In the low GC Gram-positive bacteria and Desulfovibrio desulfuricans they occur in combination with the methyl accepting chemotaxis receptor proteins, with or without the HAMP domain [ 29 30 ] .

In bacteria, the HNOB domains occurring in the chemotaxis receptors form a well-supported monophyletic group, while the remaining standalone forms are paraphyletic (Fig.

In bacteria the HNOB domain occurs either as a standalone version or fused to the HAMP and methyl accepting domains of chemotaxis receptors.

Migration assay Cell migration was assessed in 24-well chemotaxis chambers fitted with 5.

Therefore in this study we assessed the effects of mactinin on monocyte chemotaxis in vitro .

Conclusion We conclude that mactinin is present in arthritic synovial fluid in levels that can promote mononuclear phagocyte chemotaxis and maturation.

Measurement of chemotaxis Chemotactic responsiveness of lung neutrophils towards bacterially-derived n-formyl-methionyl-leucyl-phenylalanine (fMLP) was quantified using a Neuroprobe 48-microwell chemotaxis chamber and 5-µm pore size Nucleopore filters [ 12 13 ] .

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