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Sentences for "chimeras "
We constructed chimeras by placing the Cas SD at either the N-terminus or C-terminus of the Src kinase domain.
Subsequently, in all our experiments reported, we used chimeras of the Cas SD fused to the C-terminus of the Src kinase domains.
Experiments used the chimeras expressed either via the pJ3H vector, or the HA-tagged chimera subcloned into the expression vector pMEXneo [ 48 ] .
Tyrosine phosphorylation of chimeras and other substrates were determined following immunoprecipitation using the indicated antibodies and probing with RC20-HRP anti-phosphotyrosine antibodies.
Expression of the chimeras was determined with anti-HA antibodies.
Author's contributions AA carried out the construction of the Src/Cas chimeras, and characterized the chimeras with the help of MK.
AA also performed JNK assays in transient transfections, analyzed the association of the chimeras with crk and drafted the manuscript.
Studies of Rab24/Rab1B chimeras indicate that the unusual cytopathic effects induced by the D123I mutant require sequence elements within the Rab24 C-terminal hypervariable targeting domain.
Later fate mapping studies using quail-chick chimeras show extensive cranial neural crest contribution to this tissue [ 6, 7].
Although syk -deficient mice die perinatally, analysis of radiation chimeras reconstituted with fetal liver from syk -deficient mice has demonstrated a block in the transition from proB cells to preB cells, indicating that signal transduction through Syk is required for early B cell development [ 35 36 ] .
First, PcrG-GAL4-AD and LcrV-GAL4-BD chimeras were coexpressed in a yeast two-hybrid assay.
This region was identified using chimeras of ?
1S and ?
1C [ 16 ] .
Targeted ES cell clones were injected into C57Bl/6J blastocysts and chimeras obtained.
But the rise of Hamiltonian thinking coincided with the eclipse of classical cytogenetics in favor of the molecular biology of model organisms, and these remarkable little chimeras have languished in undeserved obscurity.
How have scale insects suppressed the conflicts that normally tear chimeras apart?
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