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Cell extracts were prepared and chloramphenicol acetyl transferase (CAT) assays were performed on extracts containing equivalent cell protein as previously described [ 33].

For studies of the transcriptional activation properties of the fusion proteins, 1 µg of the C/EBP-TATA vector [ 45 ] was co-transfected and transcriptional activity was assessed by measuring the amount of chloramphenicol acetyltransferase reporter expressed with and without C/EBP?

expression [ 45 ] .

When bean plants exposed to intermittent light were treated with chloramphenicol to inhibit synthesis of proteins on chloroplast ribosomes, Chl b and LHCPs accumulated in parallel with no increase in synthesis of total Chl [ 10 ] .

High concentrations of chloramphenicol caused strong suppression of total Chl synthesis when dark-grown algal cells were exposed to light, possibly by inhibition of Mg-chelatase [ 13 ] in addition to chloroplast protein synthesis.

In this vector, the first cistron, encoding chloramphenicol acetyltransferase (CAT), is proximal to the mRNA's cap structure and therefore expected to be translated by the conventional cap-dependent translation mode.

CAT enzyme activity was determined using 14C-labeled chloramphenicol as described previously [ 38 ] .

These isolates were resistant to tetracyclines in 17% of cases, to chloramphenicol in 18.

Transient transfection and chloramphenicol acetyltransferase assays Transient transfections of normal dermal fibroblasts were performed with 20 µg of the -353 fragment of ?

2(I) collagen (COL1A2) promoter linked to the chloramphenicol acetyltransferase (CAT) reporter gene by the calcium phosphate/DNA coprecipitation technique as described elsewhere [ 24].

Assays of transient transfection and chloramphenicol acetyltransferase Transient transfections of normal dermal fibroblasts were performed by the calcium phosphate/DNA coprecipitation technique as described previously [ 24].

Direct BAC end sequencing For sequencing, BAC DNA was isolated from a 100 ml LB culture containing 17 mg/l chloramphenicol using a QIAGEN Plasmid Midi Kit (Valencia, CA), following the manufacturer's instruction.


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