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Sentences for "chorionic "
Binding of truncated USF2 to a HLH element in the prostaglandin G/H synthase 2 (PGHS-2) promoter was abolished in extracts from cells stimulated by human chorionic gonadotrophin (hCG) which signals through cAMP [ 32 ] .
List of abbreviations C, catalytic subunit, cAMP, cyclic adenosine monophosphate, CAT, chloramphenicol acetyl transferase, C/EBP, CAAT/enhancer binding protein, ChoRE, carbohydrate response element, CMV, cytalomega virus, 8-CPT-cAMP, 8-(4-chlorophenyl)thio-cAMP, CREB, cAMP-response element binding protein, EMSA, electrophoretic mobility shift assay, FAS, fatty acid synthase, FSH, follicle stimulating hormone, GIRE, glucose/insulin response element, hCG, human chorionic gonadotrophin, HLH, helix-loop-helix, IRE, insulin response element, MHC, major histocompability complex, PGHS, prostaglandin G/H synthase, PKA, cAMP-dependent protein kinase, R, regulatory subunit, USF, upstream stimulating factor, WB, Western blot.
They were co-expressed with the luteinizing hormone (LH) receptor in HEK 293 cells and cAMP accumulation was measured in both the presence and absence of the LH receptor agonist, human chorionic gonadotropin (hCG).
5 ng/mL human chorionic gonadotropin (hCG), as described previously.
Women of childbearing potential had to have a negative serum ß-human chorionic gonadotropin (HCG) pregnancy test at screening, and had to be willing to use an adequate method of contraception during the study.
Chloride channels or chloride ions are known to influence cAMP-stimulated steroidogenesis by adrenal cells [ 29 ] , human chorionic gonadotropin (hCG)-stimulated steroidogenesis by amphibian follicle-enclosed oocytes [ 56 ] , as well as LH-stimulated steroidogenesis in rat Leydig cells [ 29 ] , MA-10 cells [ 59 ] , and chicken GC [ 35 ] .
List of Abbreviations 4-aminopyridine (4-AP), analysis of variance (ANOVA), 8-(4-chlorophenylthio)adenosine-3',5'-cyclic monophosphorothioate (8-CPT-cAMP), 4',4' diisothicyanato-stilbene-2-2'-disulfonic acid (DIDS), bis-(1,3-dibutylbarbituric acid)trimethine oxonol (DiBAC 4 (3)), 5?
-dihydrotestosterone (5?
-DHT), Dulbecco's modified eagle's medium (DMEM), enhanced chemiluminescence (ECL), ethylenediaminetetraacetic acid (EDTA), fetal bovine serum (FBS), follicle stimulating hormone (FSH), granulosa cells (GC), human chorionic gonadotropin (hCG), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), 3ß-hydroxysteroid dehydrogenase (3ß-HSD), 3-isobutyl-1-methylxanthine (IBMX), least significant difference (LSD), luteinizing hormone (LH), mitogen activated protein kinase (MAPK), 1,3-Benzenedicarboxylic acid, 4,4'-[1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-, tetrakis [(acetyloxy)methyl] ester (PBFI-AM), phosphate-buffered saline (PBS), proliferating cell nuclear antigen (PCNA), protein kinase A (PKA), sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), steroidogenic acute regulatory protein (StAR), tris-buffered saline (TBS).
Results ERß immunoreactivity in chorionic villi (CV) and placental membranes (Figures 1and 2) We used dual color immunohistochemistry without hematoxylin counterstain to show placental mesenchymal cells and epithelial structures (left column, Fig.
Dual color immunohistochemistry for ER?
and ERß immunoreactivity in chorionic villi (Figure 3) The relationship between ER?
and ERß immunoreactivity in CV was also investigated by dual color immunohistochemistry (Fig.
The expression of ~64 kDa ERß is most prominent in early culture (EC), protein extracts from chorionic villi show distinct ~52 kDa ERß variant (dotted arrow indicates adjacent ~53 kDa band), and trophoblast cultures show ~56 kDa band identified with E12 antibody in particular.
We also utilized frozen blocks and protein extracts from human placental chorionic villi, and protein extracts from trophoblast cultures and cultured amniotic fibroblasts and placental mononuclear cells [ 46 ] .
Placental chorionic villi (CV, lane 8) also showed six distinct bands similar to those detected in the rat, human and porcine ovaries.
Human chorionic villi and placental membranes (Figure 6) In normal placentae at term, the LHR immunoreactivity in mature chorionic villi was found in mononucleated cytotrophoblast cells under the syncytial layer (arrowheads, Fig 6A) and villous vascular sinusoids (v), but most of the syncytiotrophoblast was virtually unstained (white asterisks vs.
Figure 6Gshows LHR immunostaining of syncytium (black asterisk) and lack of vascular LHR (white arrowhead), resembling immature chorionic villi (immature placental phenotypes, characteristic for intrauterine growth retardation and maternal diabetes [ 52 ] - note lack of dilatation of villous sinusoids).
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