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Sentences for "chromatograms "
The unaffected and mixed reactions were then run at pre-determined temperatures (see Additional file A: Primer sequences and DHPLC temperatures Excel spreadsheet) on the WAVE ®system and the resultant chromatograms compared for variation in shape or retention time.
Figure 4adepicts typical chromatograms for samples in which no variation from unaffected control is detectable.
We manually inspected chromatograms for high-quality discrepancies in the genomic sequence and found none, indicating that the edited bases are not due to population heterozygosity.
However, careful analysis of the exon 20 sequence chromatograms in both forward and reverse directions from this patient's lung biopsy specimen obtained after disease progression on gefitinib demonstrated an additional small peak at nucleotide 2369, suggesting a C›T mutation (Figure 2A, upper panels, Table 1).
Sequencing chromatograms of DNA from four of four clones showed both the 2369 C›T and 2573 T›G mutations, confirming that both mutations were in the same allele (data not shown).
The ratio of mutant to wild-type products was higher in the cells from the pleural fluid, consistent with the higher peaks seen on the chromatograms from direct sequencing of exon 20 (see Figure 2A).
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