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Sentences for "chromatographic "
All the chromatographic separations were carried out at 4°C.
Identification of native parasitic PP5 To identify whether PfPP5 is actually expressed in the parasite, cell-free extracts of the asynchronous erythrocytic stage parasites were subjected to standard chromatographic procedures that had been previously optimized for human PP5 [ 18 ] .
As mentioned, the activity peaks always coincided with a ~69 kDa band in parallel Western analyses of the chromatographic fractions.
6 mm, 5 µm, Supelco, Bellefonte, PA), and analytes were separated using the ion-pair chromatographic method.
This was accomplished by liquid-solid extraction and liquid-gel chromatographic techniques to isolate the phytoestrogen fractions using standard assay methods with internal controls [ 28 ] .
However, our attempts to purify the native PfPP1 enzyme by chromatographic procedures resulted in only small amounts of activity, probably due to rapid inactivation during fractionation.
The combined hexane layers were evaporated to dryness under a stream of nitrogen and the extracted cholesterol was dissolved in hexane for gas chromatographic analysis.
All biochemical examinations (serum total cholesterol, LDL-cholesterol, HDL-cholesterol, triglycerides and blood glucose) were measured using chromatographic enzymic method in a Technicon automatic analyzer RA-1000.
Following chromatographic separation of unincorporated 35SO 4 2-using PD-10 columns (Pharmacia, Uppsala, Sweden), the levels of radiolabeled GAGs released onto the culture media or recovered by alkaline extraction were quantitated by scintillation counting [ 24 ] .
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