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Sentences for "chromogen "
USA) and H 2 O 2 was used as chromogen and substrate, respectively.
The blots were developed using DAB (diaminobenzimidine) and H 2 O 2 as chromogen and substrate, respectively.
Immunoreactivity of the primary antibody was then labeled either with the chromogen diamino benzidine (DAKO Diagnostika, Hamburg) or with a fluorophore-coupled secondary antibody.
Detection of Biotin Chromogen Peroxidase was demonstrated using either DAB [3,3'-diaminobenzidene] (DAKO) or AEC [3-amino-9-ethylcarbazole] (Sigma).
AEC was the chromogen of choice for clean background.
After washing with PBS, the antibodies were detected using reagents in the Vectastain ABC Elite Kit (Vector Labs, Burlingame, USA), the sections were treated with biotinylated goat anti-rabbit IgG (at 1/200 dilution) for 30 min, rinsed with PBS, incubated with streptavidin-conjugated peroxidase for 30 min, washed with PBS, then treated with the chromogen 3,3'-diaminobenzidine for 5 min.
Slides were developed with a substrate chromogen solution until a brown reaction product was observed, then counterstained with hematoxylin and coverslipped.
Other reagents, including the secondary antibody, detection kits, and chromogen kits, were purchased from Vector Laboratories, Inc (Burlingame, CA).
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