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Allele specificity of circularization is dependent upon two factors: 1) favorable hybridization of the matching OCP because of the correct base pair formed at the 3' end and also because of base stacking between the 3' and 5' annealed ends, and 2) specificity of the ligase for correctly base paired ends.

ERF defines a superfamily of SSAPs that are evolutionarily distinct from the RecT/Redß super family The ERF protein of phage P22 is involved in the circularization of the linear dsDNA phage genome upon entry into the host cell [ 21 22 23 ] .

The OCP ligation/ERCA method is based on ligation dependent circularization of allele specific DNA probes followed by exponential rolling circle amplification of the circularized probes [ 4 5 ] .


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