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Results IP 3Receptor Isotypes Forty micron thick rat sections containing circumvallate papillae were exposed to either anti-IP 3 R1, anti-IP 3 R2, or anti-IP 3 R3 antibodies and appropriate secondary antibodies.
Laser scanning confocal microscopy of the resultant sections was used to examine the distribution of IP 3 receptor immunoreactivity in rat circumvallate taste buds.
We also compared IP 3 R3 IR with ?
13 IR in rat and mouse circumvallate taste buds.
Single label Immunocytochemistry Sections from rat circumvallate papillae were incubated with primary antibodies overnight at 4°C.
Thus T1R2 and T1R3 were coexpressed in circumvallate and foliate taste buds but not in fungiform papillae [ 6 ] , while sweeteners elicited stronger responses in the CT than in the NG.
The circumvallate are shaped like an inverted V and are found only near the root of the tongue, the largest of this class of papillæ have other very small papillæ upon their surfaces.
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