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Sentences for "clonal "
Next, adeno-RGS3 was isolated by clonal selection in HEK 293 cells followed by a second PCR to confirm the presence of RGS3 cDNA.
Here we have explored the relationship of SV40 large T antigen (Tag) expression as a function of cell density and cell cycle duration in clonal populations of Tag-immortalized mouse astrocytes.
Results Characterization of tkT lines We have previously characterized clonal mouse astrocyte cell lines, immortalized by transduction of SV40 large T antigen expressed from the MoMuLV LTR [ 6 13 ] .
In this study, the expression potential of cell lines was not related to proliferation related changes in expression of Tag, emphasizing that clonal variation for whatever reason (which includes promoter strength) accounts for the large range of htk related expression.
This was not true for clonal or polyclonal transductants that expressed Tag from the MoMuLV LTR, which supports a high level of expression (unpublished observations).
However, as discussed above, Tag levels in clonal cell lines produced with the weak htk promoter and generated from slowly growing primary cells are related to clonality.
Conclusions These data show quantitatively that the Tag expression potential of introduced Tag varies over a wide range depending on the vector used and clonal variation.
These GFP-MHCK expressing cells were able to sporulate and grow in suspension, indicating that at the expression level of these clonal cell lines, the expression of GFP-MHCKs in the AX2 cells does not detectably change myosin II expression or function.
The resulting plasmid, pTX-MKC2, was transformed into the cell line Ax2 and clonal cell lines were selected with G418 (8 µg/ml) in HL-5 medium.
This is important because each round of clonal selection increases the likelihood of losing germline competency of the ES cells.
However, if a line of "transgenic" ES cells can be established then it is possible to generate clonal embryos directly from these ES cells by aggregating them with tetraploid embryos.
It has also been proposed that the CD8 +CD28 -T cell in the HIV +state is derived from the CD8 +CD28 +cells by clonal expansion induced by massive antigenic stimulus (HIV proteins) since both showed the same epitope repertoire [ 26 ] .
Likewise in the aged state, we can speculate that chronic viral and bacterial exposure over a lifetime may induce the clonal expansion of the CD8 +CD28 -T cell.
It is known that during clonal selection in the thymus, immature CD4, CD8 double positive cells develop into single positive CD4 or CD8 cells according to the relative affinity of their TCR to class II or class I molecules respectively [ 5 6 ] .
They progress to mature ball-forms just as clonal fibers grown in isolation do.
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