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Sentences for "clonally "
The Linker-tkT lines (tkT) were either isolated as clones after G418 selection (as previously described) [ 6 ] or frozen immediately after selection (pooled clones) and clonally selected at a later time.
To identify potential downstream targets of MeCP2 modulation, we have compared global gene expression patterns in four matched pairs of clonally derived mutant or wild-type MECP2-expressing fibroblast cell strains from girls with Rett syndrome, and in five wild-type and four mutant MeCP2-expressing lymphoblastoid cell lines, by hybridizing Affymetrix U95 (A-E) oligonucleotide microarrays.
The use of clonally derived fibroblasts eliminated the mosaic X inactivation seen in tissues from most females with Rett syndrome.
The use of lymphoblastoid cell lines, while not all clonally derived, expanded the scope of genes to be assayed beyond those expressed in skin fibroblasts.
From four original fibroblast samples, we generated clonally derived cell strains that expressed only the mutant MeCP2 or only the wild-type MECP2 allele, but not both.
In order to confirm that these clonally expanded B cells were receiving ongoing antigenic stimulation and not limited solely to the memory compartment, we employed the cDNA-based assay to distinguish clonal expansions of activated B cells from resting (memory) cells.
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