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Sentences for "coculture "
With respect to osteoclast formation, IFN-?
is known to potently inhibit RANKL-mediated osteoclastogenesis in both spleen-derived macrophage cultures and bone marrow coculture systems [ 19 20 21 ] .
Direct coculture of hESMPCs with C2C12 cells led to the formation of hESMPC-derived myotubes, as visualized by expression of human nuclear antigen (Figure 3B), similar to those formed by host C2C12 cells.
Human cell contribution to myotubes in coculture was confirmed by expression of human muscle-specific transcripts such as MyoD, myosin heavy chain IIa , and myogenin (data not shown).
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